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a Children's Hospital, University of Würzburg,
Würzburg, Germany, b Pettenkofer-Institute,
University of Munich, Munich, Germany
Correspondence to: Dr H-I Huppertz, Children's Hospital, University of Würzburg, Josef-Schneider-Str 2, 97080 Würzburg, Germany.
Accepted for publication 7 August 1997
OBJECTIVE
Analysis of the interaction of
enteropathogenic bacteria with HLA B27 transfected murine fibroblasts
showed a specific influence of HLA B27 on microbial invasiveness. This
possible novel mechanism for the action of HLA B27 should be verified
by using endogenous HLA B27 positive and negative human fibroblasts as
a model for the direct interaction of arthritogenic bacteria and host cells.
METHODSFibroblasts were obtained from healthy
donors positive or negative for HLA B27; cultivated as monolayers; and
infected with Salmonella enterica serovar enteritidis.
RESULTSInvasion and survival of bacteria in human
cells was not influenced by the presence of HLA B27. Enhancement of HLA
class I molecule expression by treatment of cultures with interferon
gamma decreased invasion and survival of bacteria in both HLA B27
positive and negative cells. After disappearance of live bacteria
lipopolysaccharide antigens persisted within cells.
CONCLUSIONEndogenous HLA B27 does not modulate
the direct interaction of Salmonella with human cells.
Non-professional phagocytes are able to limit bacterial survival in
cells, and interferon gamma accelerates killing of bacteria, but
arthritogenic antigens persist after disappearance of live bacteria.
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