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Ann Rheum Dis 1997;56:262-267 ( April )

Extended reports

Characterisation of fibroblast-like cells in pannus lesions of patients with rheumatoid arthritis sharing properties of fibroblasts and chondrocytes Chengsen Xue,a Masayoshi Takahashi,a Tomoko Hasunuma,a Hiroyuki Aono,a Kazuhiko Yamamoto,a Shinichi Yoshino,b Takayuki Sumida,a Kusuki Nishiokaa

a Department of Rheumatology and Immunology, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki, Japan , b Division of Rheumatology, Nippon Medical College, Tokyo, Japan

Correspondence to: Dr K Nishioka, Department of Rheumatology and Immunology, Institute of Medical Science, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki 216, Japan.

Accepted for publication 27 November 1996

OBJECTIVE---To better understand the characteristics of synoviocytes located in the rheumatoid arthritis (RA) pannus.
METHODS---One cell line, termed PSC, was cloned from RA pannus lesions. Phenotypic analysis was done by contrast microscopy, indirect immunostaining, and safranin O staining. Transcription of several protooncogenes and matrix degrading enzymes was evaluated. The expression of mRNA for collagen II was detected by in situ hybridisation. The ability of anchorage independent growth was assessed by soft agarose culture.
RESULTS---PSCs showed a high transcription of protooncogenes c-fos, c-myc and c-jun. They also expressed mRNA for matrix degrading enzymes, such as collagenase, cathepsin B, and cathepsin L. Anchorage independent growth assay demonstrated that PSCs formed colonies in soft agar culture. Phenotypic analysis showed that this fibroblast-like PSC was stained intensely with anti-vimentin and anti-fibroblast antibody. In situ reverse transcriptase assay showed that the cell line expressed type II collagen mRNA.
CONCLUSION---Alternative fibroblast-like cells were identified in the pannus lesion of RA sharing properties of fibroblasts and chondrocytes. These findings suggest that this fibroblast-like cell derived from pannus lesions may contribute to the destruction of the cartilage in RA.

© 1997 by Annals of the Rheumatic Diseases


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Actin cytoskeleton dynamics linked to synovial fibroblast activation as a novel pathogenic principle in TNF-driven arthritis
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