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B activity in T cells from patients with
rheumatic diseases: A preliminary report
a Departamento
de Medicina, Rheumatology Unit, Hospital Universitario "Reina
Sofía", Spain, b Departamento de Fisiología e Inmunología, Facultad de
Medicina, Universidad de Córdoba, Spain
Correspondence to: Professor E Collantes-Estévez, Departamento de Medicina, Unidad de Reumatología. Facultad de Medicina, Avda Menéndez Pidal s/n, E-14071 Córdoba, Spain.
Accepted for publication 23 September 1998
OBJECTIVE
The NF-
B/Rel family of transcription
factors regulates the expression of many genes involved in the immune
or inflammatory response at the transcriptional level. The aim of this
study was to determine whether distinctive patterns of NF-kB activation are seen in different forms of joint disease.
METHODSThe DNA binding activity of these
nucleoproteins was examined in purified synovial and peripheral T cells
from patients with various chronic rheumatic diseases (12: four with
rheumatoid arthritis; five with spondyloarthropathies; and three with osteoarthritis).
RESULTSElectrophoretic mobility shift assays
disclosed two specific complexes bound to a NF-B specific
32P-labelled oligonucleotide in nucleoproteins extracted
from purified T cells isolated from synovial fluid and peripheral blood
of patients with rheumatoid arthritis. The complexes consisted of
p50/p50 homodimers and p50/p65 heterodimers. Increased NF-kB binding to DNA in synovial T cells was observed relative to peripheral T cells. In
non-rheumatoid arthritis, binding of NF-B in synovial T cells was
exclusively mediated by p50/p50 homodimers.
CONCLUSIONOverall, the results suggest that
NF-B may play a central part in the activation of infiltrating T
cells in chronic rheumatoid arthritis. The activation of this nuclear
factor is qualitatively different in rheumatoid synovial T cells to
that in other forms of non-rheumatoid arthritis (for example,
osteoarthritis, spondyloarthropathies).
B;
synovial T cells;
rheumatoid arthritis;
spondyloarthropathy;
osteoarthritis
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