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a Departments of
Immunology and Medicine, b and Department of Internal Medicine, c St Marianna University School of Medicine,
Kawasaki, Kanagawa, Japan Department of
Orthopaedic Surgery, Osaka University School of Medicine, Osaka, Japan
Correspondence to: Dr T Sakane, Departments of Immunology and Medicine, St Marianna University School of Medicine, 2-16-1, Sugao, Miyamae-ku, Kawasaki, Kanagawa 216, Japan.
Accepted for publication 21 May 1998
OBJECTIVE
To elucidate
possible roles of several transcription factors in the pathogenesis of
rheumatoid arthritis (RA), the transcription factor expression in RA
synovial tissue and their contribution to RA synovial cell functions
were studied.
METHODSSingle
cell suspension of dissociated synovial tissue was cultured to induce
in vitro tissue outgrowth of RA synovial cells. Transcription factors
were immunohistochemically identified in RA synovial tissue obtained by
joint surgery and in the in vitro tissue outgrowth, and confirmed
by western blotting and gel shift assays.
RESULTSImmunohistochemical
examination of RA synovial tissue revealed simultaneous expression of
various transcription factors (NF-
B, c-Jun (a component of AP-1),
cAMP responsive element binding protein (CREB), and OCT-1). The same
set of transcription factors was expressed in the in vitro tissue
outgrowth of RA patients. The early passage RA synovial cells were
treated with interleukin 1
(IL1) and confirmed translocation of
transcription factors into the nucleus by western blotting, and their
DNA binding activity by gel shift assays.
CONCLUSIONThis study
emphasises the importance of the simultaneous expression of several
transcription factors for the hyperactivity of RA synovial cells that
leads to tissue outgrowth.
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